human soluble cd5 elisa kit Search Results


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OriGene human soluble cd5 elisa kit
Figure 1. Fratricide-resistant <t>CD5</t> CART cells internalize and lose detectable CD5 (A) Schematic of the CD5.CAR and DCD5.CAR. (B) Representative histograms and MFI of surface CD5 expression in control or CD5 knocked-out (CD5KO) primary human T cells non-transduced (NT) or gammaretrovirally transduced with CD19.CAR, CD5.CAR, and DCD5.CAR constructs. Representative data from 6 individual healthy donors from 2 experiments. p values calculated using one-way ANOVA with Dunnett’s correction. Data are represented as mean ± SD. (C) CD5 protein expression in control or CD5KO primary human T cells expressing CD19.CAR, CD5.CAR, and DCD5.CAR. Representative data from 2 donors. Data are represented as mean ± SD. (D) Schema of fluorescent mScarlet-tagged CD5, fluorescent mEmerald-tagged DCD5.CAR, or bi-cistronic DCD5.CAR with CD5.mScarlet (top) and the timeline of live-imaging microscopy experiment (bottom). (E) Maximal-projection images from live-imaging fluorescent microscopy of T cells serially transduced with CD5.mScarlet and DCD5.CAR-mEmerald. mScarlet localization (top). mEmerald localization (middle). Differential interference contrast (DIC) overlay with mScarlet and mEmerald (bottom). (F) Z-slice images of mScarlet localization in T cells when CD5.mScarlet is expressed in the absence (top) or presence of DCD5.CAR (bottom).
Human Soluble Cd5 Elisa Kit, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+soluble+cd5+elisa+kit/Human+soluble+CD5+ELISA+Kit/pm38986621-246-10-18
Average 92 stars, based on 1 article reviews
human soluble cd5 elisa kit - by Bioz Stars, 2026-09
92/100 stars
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Human soluble CD5 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit.
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Human soluble CD5 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human CD5 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
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Human soluble CD5 AccuSignal ELISA Kit - KOA0713
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Image Search Results


Figure 1. Fratricide-resistant CD5 CART cells internalize and lose detectable CD5 (A) Schematic of the CD5.CAR and DCD5.CAR. (B) Representative histograms and MFI of surface CD5 expression in control or CD5 knocked-out (CD5KO) primary human T cells non-transduced (NT) or gammaretrovirally transduced with CD19.CAR, CD5.CAR, and DCD5.CAR constructs. Representative data from 6 individual healthy donors from 2 experiments. p values calculated using one-way ANOVA with Dunnett’s correction. Data are represented as mean ± SD. (C) CD5 protein expression in control or CD5KO primary human T cells expressing CD19.CAR, CD5.CAR, and DCD5.CAR. Representative data from 2 donors. Data are represented as mean ± SD. (D) Schema of fluorescent mScarlet-tagged CD5, fluorescent mEmerald-tagged DCD5.CAR, or bi-cistronic DCD5.CAR with CD5.mScarlet (top) and the timeline of live-imaging microscopy experiment (bottom). (E) Maximal-projection images from live-imaging fluorescent microscopy of T cells serially transduced with CD5.mScarlet and DCD5.CAR-mEmerald. mScarlet localization (top). mEmerald localization (middle). Differential interference contrast (DIC) overlay with mScarlet and mEmerald (bottom). (F) Z-slice images of mScarlet localization in T cells when CD5.mScarlet is expressed in the absence (top) or presence of DCD5.CAR (bottom).

Journal: Cell reports. Medicine

Article Title: Chimeric antigen receptor-induced antigen loss protects CD5.CART cells from fratricide without compromising on-target cytotoxicity.

doi: 10.1016/j.xcrm.2024.101628

Figure Lengend Snippet: Figure 1. Fratricide-resistant CD5 CART cells internalize and lose detectable CD5 (A) Schematic of the CD5.CAR and DCD5.CAR. (B) Representative histograms and MFI of surface CD5 expression in control or CD5 knocked-out (CD5KO) primary human T cells non-transduced (NT) or gammaretrovirally transduced with CD19.CAR, CD5.CAR, and DCD5.CAR constructs. Representative data from 6 individual healthy donors from 2 experiments. p values calculated using one-way ANOVA with Dunnett’s correction. Data are represented as mean ± SD. (C) CD5 protein expression in control or CD5KO primary human T cells expressing CD19.CAR, CD5.CAR, and DCD5.CAR. Representative data from 2 donors. Data are represented as mean ± SD. (D) Schema of fluorescent mScarlet-tagged CD5, fluorescent mEmerald-tagged DCD5.CAR, or bi-cistronic DCD5.CAR with CD5.mScarlet (top) and the timeline of live-imaging microscopy experiment (bottom). (E) Maximal-projection images from live-imaging fluorescent microscopy of T cells serially transduced with CD5.mScarlet and DCD5.CAR-mEmerald. mScarlet localization (top). mEmerald localization (middle). Differential interference contrast (DIC) overlay with mScarlet and mEmerald (bottom). (F) Z-slice images of mScarlet localization in T cells when CD5.mScarlet is expressed in the absence (top) or presence of DCD5.CAR (bottom).

Article Snippet: Levels of soluble CD5 were measured in thawed supernatants using Human soluble CD5 ELISA Kit (Cat # EA100720, OriGene, Rockville, MD).

Techniques: Expressing, Control, Transduction, Construct, Imaging, Microscopy